MIF deficiency reduces liver damage and alters liver cell composition during <i>T. brucei</i> infection.

<p>(A) Liver weight (left panel), serum ALT (middle panel) and AST (right panel) levels in <i>Mif</i>^−/− (white bars) and WT (black bars) C57Bl/6 mice at day 25 p.i. (B) Representative liver immune cell gating strategy: Ly6c versus CD11b plot following gating on CD45+ and 7AAD- cells allows identifying CD11b⁺Ly6C⁺ cells (middle panel). This population was plotted in a Ly6C versus Ly6G plot to distinguish CD11b⁺Ly6C^highLy6G⁻ inflammatory monocytes and CD11b⁺Ly6C^intLy6G⁺ neutrophils (right panel). Percentage of CD11b⁺Ly6C⁺ cells within the liver CD45+ cells in WT (black bars) and <i>Mif</i>^−/− (open bars) mice (left panel). (C) Number of CD11b⁺Ly6C^highLy6G⁻ and CD11b⁺Ly6C^intLy6G⁺ cells within the liver CD45+ cells (upper left panels). The dashed line represents values in non-infected mice. Total liver CCL2, KC (CXCL1) and LIX (CXCL5) gene expression levels in <i>Mif</i>^−/− (white bars) and WT (black bars) mice at day 25 p.i. (lower panels). Gene expression levels are normalised using <i>s12</i> and expressed relatively to expression levels of non-infected mice. Results are representative of 2 independent experiments and presented as mean of 3 individual mice ± SEM (*: p-values ≤0.05, **: p-values ≤0.01).