Neuronal surface binding of MBP (10 µg/ml, 5 min) is abolished by MBP-acidic lipid pre-mixing.

<p>MBP was pre-mixed with various lipids, the mixture was used for neuronal incubation, and subsequent MBP surface staining was performed to assess MBP surface binding (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0108646#s2" target="_blank">Materials and Methods</a> for details). PtdIns: phosphatidylinositol; PS: phosphatidylserine; PA: phosphatidic acid; GM1: monosialoganglioside; GD1a: disialoganglioside; PC: phosphatidylcholine; PE: phosphatidylethanolamine.</p>a<p>: No MBP surface staining observed.</p>b<p>: MBP surface binding observed.</p>c<p>: Not done.</p><p>Neuronal surface binding of MBP (10 µg/ml, 5 min) is abolished by MBP-acidic lipid pre-mixing.