Dose-response effects of TGF-beta on TMEM16A, CFTR, e-cadherin, and vimentin in T84 cells.

<p>T84 cells were treated with TGF-beta (vehicle control, 0.1, 1.0, and10 ng/ml) for 48 h, followed by Ussing chamber and immunoblot as described in Methods. (<b>A and B</b>) CaCC (*<i>P<</i>0.003, **P<0.01) and CFTR (*<i>P<</i>0.0001) currents were reduced by TGF-beta treatment at every dose used. (<b>C and D</b>) TMEM16A (*<i>P<</i>0.01, **P<0.005) and CFTR (*<i>P<</i>0.05, **P<0.0001) expression was suppressed by TGF-beta treatment at every dose used. E-cadherin expression (<b>E</b>) was decreased (*<i>P</i><0.011) and vimentin expression (<b>F</b>) was increased (*<i>P</i><0.05) with 1 ng/ml or greater TGF-beta treatment.