Density of VGLUT1+ and VGLUT2+ axons (A, immunofluorescence assay) and protein levels of VGLUT1 and VGLUT2 (B, C, Western blot assay) in the control, CFA 1-day and 3-day pulps.

<p><b>A</b>: The density (area fraction) of VGLUT1+ pulpal axons is not significantly different between CFA 1-day or CFA 3-day groups and control, whereas the density of VGLUT2+ axons is significantly higher in the CFA 1-day, CFA 3-day groups than control. N = 3 animals in each group. *p<0.01. <b>B</b>: Representative images of the Western blot assay. <b>C</b>: Quantitative analysis of VGLUT1 and VGLUT2 protein in the dental pulp. Protein levels of VGLUT1 and VGLUT2 in the pulp are significantly higher in the CFA 1-day, CFA 3-day groups than control. This difference is bigger for VGLUT2 (3.9 and 4.3 fold higher in the CFA 1-day and CFA 3-day groups than control) than for VGLUT1 (2.9 and 3.1 fold higher in the CFA 1-day and CFA 3-day groups than control). N = 5 animals in each group. *p<0.01.