GC size, adhesion and advance of APP mutant neurons on laminin.

<p><b>A.</b> Western blots of GCPs isolated from newborn brain of wt and mutant mice. Blots were probed for APP (M_r ∼100 kDa), APLP1 (M_r = 72 kDa), and APLP2 (M_r = 87 kDa, asterisk) and for Gap43 as loading control. APLP1 and APLP2 levels were the same in wt and mutant mice. <b>B.</b> Phase contrast images of wt and mutant neurons and (<b>C</b>) TIRF images of their GCs labeled with fluorescent phalloidin. <b>D.</b> RICM images of live GCs showing close adhesions (dark) and wide contacts (bright). hAPP+ control GCs (ctrl; non-transgenic siblings) were the same as wt and, therefore, are not shown. Scale bars, 20 µm (<b>B</b>) and 10 µm (<b>C, D</b>). <b>E.</b> GC area (blue), cumulative adhesive area (green) and axon length (red) at 24 h <i>in vitro</i> for the indicated mouse strains (means ± s.e.). For statistical analysis, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064521#pone-0064521-t001" target="_blank">Tables 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064521#pone-0064521-t002" target="_blank">2</a>.