SVHRP increases BDNF expression in the adult SGZ and SVZ.

<p>Immunostaining of BDNF (red) and GFAP (green). Scale bars represent 100 µm. a. Control; b. SVHRP. A) in SGZ. B) in SVZ. LV: lateral ventricle. C) Quantification of BDNF- and GFAP- positive cells per SGZ and SVZ slice. The bar graph shows the area of BDNF- and GFAP-positive cells (mean ± SEM) in SGZ and SVZ sections. Control group <i>vs</i> SVHRP group, *<i>p</i><0.05, n = 3. D) RT-PCR of BDNF in the SVZ and SGZ. E) Quantification BDNF mRNA levels in the SVZ and SGZ. The bar graph shows the intensity of GFAP/GAPDH (mean ± SEM) in SVZ and SGZ sections. Control group <i>vs</i> SVHRP group, *<i>p</i><0.05, n = 4. F) BDNF protein levels in the SVZ and SGZ. G) Quantification of BDNF protein levels of in SVZ and SGZ. The bar graph shows the intensity of BDNF/β-actin (mean ± SEM) in SVZ and SGZ. Control group <i>vs</i> SVHRP group *<i>p</i><0.05, n = 3. H) BDNF in the in SVZ and SGZ was measured with ELISA assay. The bar graph represents amount of BDNF (mean ± SEM) in SVZ and SGZ. Control group <i>vs</i> SVHRP group <i>*p<0.05</i>, n = 4.