KDM5 directly regulates oxidative stress resistance genes.

<p>(A) Real-time PCR analyses of <i>4E-BP</i>, <i>l(2)efl</i>, <i>CG5316</i>, <i>spirit</i>, <i>Prx2540-2</i> and <i>CG10211</i> from wildtype (<i>w¹¹¹⁸</i>), <i>kdm5^K6801</i> mutant and <i>foxo²¹</i> mutant 3^rd instar larvae in 5% sucrose or 20 mM paraquat/5% sucrose for six hours (oxidative stress). (B) Schematic of the promoters of <i>4E-BP</i>, <i>l(2)efl</i>, <i>CG5316</i>, <i>spirit</i>, <i>Prx2540-2</i> and <i>CG10211</i> showing the position of the Foxo binding site (FHREs; black boxes). Primers surrounding these sites were used for ChIP analyses shown in parts C and D. (C) ChIP analyses of <i>4E-BP</i>, <i>l(2)efl</i>, <i>CG5316</i>, <i>spirit</i>, <i>Prx2540-2</i> and <i>CG10211</i>. Anti-Foxo ChIP is shown in black bars while control anti-Foxo ChIP from <i>foxo²¹</i> homozygous mutant larvae is shown in grey. Data are shown as % of input DNA. All six genes tested show significant attenuation of anti-Foxo ChIP signal (<i>p</i><<0.01). (D) ChIP analyses of <i>4E-BP</i>, <i>l(2)efl</i>, <i>CG5316</i>, <i>spirit</i>, <i>Prx2540-2</i> and <i>CG10211</i>. Anti-KDM5 ChIP is shown in black bars while control anti-KDM5 ChIP from <i>kdm5^K6801</i> homozygous mutant larvae is shown in grey. Data are shown as % of input DNA. All six genes tested show significant attenuation of anti-KDM5 ChIP signal (<i>p</i><<0.01).