The H3K4me3 demethylase activity of KDM5 is not required for it to regulate KDM5-Foxo target genes.

<p>(A) Western blot showing levels of KDM5, Foxo and the loading control histone H3 in wildtype (<i>w¹¹¹⁸</i>) and <i>kdm5</i> mutant (<i>kdm5^K6801</i>) 3^rd instar wing imaginal discs (10 discs per lane). (B) Western blot showing levels of KDM5, Foxo and histone H3 in wildtype (<i>w¹¹¹⁸</i>), and <i>foxo²¹</i> mutant 3^rd instar wing discs. (C) H3K4me3 ChIP to the FHRE region of <i>puc</i>, <i>4E-BP</i>, <i>l(2)efl</i>, <i>CG5316</i>, <i>spirit</i>, <i>Prx2540-2</i> and <i>CG10211</i> promoters in <i>lid¹⁰⁴²⁴</i>/<i>lid¹⁰⁴²⁴</i>; gKDM5/gKDM5 (blue) and <i>lid¹⁰⁴²⁴</i>/<i>lid¹⁰⁴²⁴</i>; gKDM5^JmjC*/gKDM5^JmjC* (red) larvae. * <i>p</i><0.05. IgG is included as a negative control. (D) Real-time PCR showing expression levels in wildtype (<i>lid¹⁰⁴²⁴</i>/<i>lid¹⁰⁴²⁴</i>; gKDM5/gKDM5 (black) and <i>lid¹⁰⁴²⁴</i>/<i>lid¹⁰⁴²⁴</i>; gKDM5^JmjC*/gKDM5^JmjC* (grey) 3^rd instar larvae. Data are normalized to <i>rp49</i> expression and changes to expression in demethylase inactive larvae are shown relative to <i>lid¹⁰⁴²⁴</i>/<i>lid¹⁰⁴²⁴</i>; gKDM5/gKDM5 control larvae. ** <i>p</i><0.01.