Negative control and direct 16S ddPCR versus qPCR comparisons.

<p>A) Direct comparison of the negative control for total bacterial 16S count between ddPCR and qPCR. There was a significant difference between the ddPCR and qPCR negative controls with ddPCR being on average lower. B) The linear correlation between the qPCR and ddPCR results were significant (y = 0.33× + 1.44, R² of 0.27).