A quantification correlation was obtained by measuring copy number per reaction against the assigned copy number per reaction (A). A quantification correlation was obtained by Cq value against log starting concentration for QPCR (B). The correlation coefficient (R2) assay for recombinant plasmid pUC57 in ddPCR and qPCR were 0,9998 and 0,9971, respectively.</p
<p>Repeatability and reproducibility of the <i>rbp2</i> plasmid standard curves determined via Cq va...
The tRNApyl and pylRSwt qPCR assays were tested with a tenfold serial dilution of their respective p...
<p>(A, B, and C; upper panel) For stander curves of the qPCR, the purified recombinant plasmids DNA ...
The linearity and quantification correlation of ddPCR between measured copy number per reaction and ...
<p>(<b>a</b>) Pol copy numbers measured by ddPCR and qPCR were significantly correlated (Pearson R<s...
A representative LOD and LOQ amplification curve of qPCR (A) and 1D amplitude plot of ddPCR reaction...
Quantification using ddPCR was shown in copy number due to its capability to perform absolute quanti...
<p>The calibration curves were generated by amplification of 10-fold dilutions of plasmid DNA standa...
<p>The Pearson correlation coefficient of singleplex 16S plasmid DNA regression curve (y = 0.7702x+2...
A representative 1D amplitude plot of ddPCR reactions (A) and amplification curve of qPCR (B). ddPCR...
<p>Panels (A) – (C) show usRNA data, and panels (D) – (F) show msRNA data. (A, D) Quantification of ...
The assessment was carried out by twelve different laboratories using the pUC57 plasmid with a conce...
<p>The <i>black circle</i> indicates the correlation between the qPCR assay from the control samples...
<p>Samples B-1 and B-2 are bacterial suspensions in high concentration. Samples P-1 –P-6 are positiv...
<p>A: Representative amplification plots of plasmid containing <i>JAK2</i> exon 12 mutation diluted ...
<p>Repeatability and reproducibility of the <i>rbp2</i> plasmid standard curves determined via Cq va...
The tRNApyl and pylRSwt qPCR assays were tested with a tenfold serial dilution of their respective p...
<p>(A, B, and C; upper panel) For stander curves of the qPCR, the purified recombinant plasmids DNA ...
The linearity and quantification correlation of ddPCR between measured copy number per reaction and ...
<p>(<b>a</b>) Pol copy numbers measured by ddPCR and qPCR were significantly correlated (Pearson R<s...
A representative LOD and LOQ amplification curve of qPCR (A) and 1D amplitude plot of ddPCR reaction...
Quantification using ddPCR was shown in copy number due to its capability to perform absolute quanti...
<p>The calibration curves were generated by amplification of 10-fold dilutions of plasmid DNA standa...
<p>The Pearson correlation coefficient of singleplex 16S plasmid DNA regression curve (y = 0.7702x+2...
A representative 1D amplitude plot of ddPCR reactions (A) and amplification curve of qPCR (B). ddPCR...
<p>Panels (A) – (C) show usRNA data, and panels (D) – (F) show msRNA data. (A, D) Quantification of ...
The assessment was carried out by twelve different laboratories using the pUC57 plasmid with a conce...
<p>The <i>black circle</i> indicates the correlation between the qPCR assay from the control samples...
<p>Samples B-1 and B-2 are bacterial suspensions in high concentration. Samples P-1 –P-6 are positiv...
<p>A: Representative amplification plots of plasmid containing <i>JAK2</i> exon 12 mutation diluted ...
<p>Repeatability and reproducibility of the <i>rbp2</i> plasmid standard curves determined via Cq va...
The tRNApyl and pylRSwt qPCR assays were tested with a tenfold serial dilution of their respective p...
<p>(A, B, and C; upper panel) For stander curves of the qPCR, the purified recombinant plasmids DNA ...