Changes in iNOS expression in glaucomatous optic nerve at 7 days, post injury.

<p>The optic nerve (non-myelinated; 2 mm post globe) of Brown Norway rats was removed 7-days post glaucomatous injury. Contralateral optic nerve was used as the normal control. Cryosections were immunostained by anti-iNOS antibodies as indicated horizontally. Ocular treatments are indicated vertically. Green color indicates staining for iNOS and blue nuclei for DAPI. There was no positive staining when primary antibodies were omitted (data not shown). Data shown here are representations of at least four independent experiments. A total of 10 animals were used in this experiment. Comparable staining for iNOS was seen in at least 4 animals in each treatment group.