<p>PGs of newly emerged females were dissected, and total RNA was extracted for qPCR analysis. Rp49 gene was used as the housekeeping gene for normalization. The data represent the mean values ± SE of three biological replicates. Significance of comparisons are marked with *** (<i>p</i><0.01) as determined by the Student’s <i>t</i>-test.</p
<p>Six genes, which were identified as differentially expressed by RNA-Seq, were selected for valida...
Data were normalized to gapdh as the reference gene and presented as a fold change between the Selec...
<p>(A) 13 genes expression from qRT-PCR. Results respresent mean + S.E.M. (N = 5) (B) 13 genes expre...
RNA extracted from denodulated roots or isolated roots. Quantification by RT-qPCR is given relative ...
<p>Note: qPCR data. R – relative mRNA level (n-fold increase), in parenthesis a range of mRNA level ...
<p>Data from qPCR are shown as means ± standard error (SE) of three replicates. RPKM from RNA-seq ar...
<p>Expression levels of 26 randomly selected genes in the four samples used in this study were detec...
Five genes were selected to verify RNA-Seq results using qRT-PCR. Graphs on the left (A, C, E, G, I,...
<p>Line diagrams represent the expression patterns of selected unigenes from the <i>M</i>. <i>cichor...
<p>PGs were collected at different developmental stages (−72, 0, and 72 h), and total RNA was extrac...
<p>qPCR ct values are the mean of 3 replicates. ct values were determined using the delta-delta meth...
<p>Normalized gene expressions levels comparing diapausing and non-diapausing <i>D</i>. <i>montana</...
<p>A) Relative expression distribution (Y-axis) of ten selected genes is shown as a ratio comparing ...
<p>Bar plots of Log2ChangeFold of the subset of genes chosen to validate the RNASeq (red) pattern of...
<p>Gene expression levels as determined by qPCR (Rn) in normothermic (NT) and hyperthermic (HT) anim...
<p>Six genes, which were identified as differentially expressed by RNA-Seq, were selected for valida...
Data were normalized to gapdh as the reference gene and presented as a fold change between the Selec...
<p>(A) 13 genes expression from qRT-PCR. Results respresent mean + S.E.M. (N = 5) (B) 13 genes expre...
RNA extracted from denodulated roots or isolated roots. Quantification by RT-qPCR is given relative ...
<p>Note: qPCR data. R – relative mRNA level (n-fold increase), in parenthesis a range of mRNA level ...
<p>Data from qPCR are shown as means ± standard error (SE) of three replicates. RPKM from RNA-seq ar...
<p>Expression levels of 26 randomly selected genes in the four samples used in this study were detec...
Five genes were selected to verify RNA-Seq results using qRT-PCR. Graphs on the left (A, C, E, G, I,...
<p>Line diagrams represent the expression patterns of selected unigenes from the <i>M</i>. <i>cichor...
<p>PGs were collected at different developmental stages (−72, 0, and 72 h), and total RNA was extrac...
<p>qPCR ct values are the mean of 3 replicates. ct values were determined using the delta-delta meth...
<p>Normalized gene expressions levels comparing diapausing and non-diapausing <i>D</i>. <i>montana</...
<p>A) Relative expression distribution (Y-axis) of ten selected genes is shown as a ratio comparing ...
<p>Bar plots of Log2ChangeFold of the subset of genes chosen to validate the RNASeq (red) pattern of...
<p>Gene expression levels as determined by qPCR (Rn) in normothermic (NT) and hyperthermic (HT) anim...
<p>Six genes, which were identified as differentially expressed by RNA-Seq, were selected for valida...
Data were normalized to gapdh as the reference gene and presented as a fold change between the Selec...
<p>(A) 13 genes expression from qRT-PCR. Results respresent mean + S.E.M. (N = 5) (B) 13 genes expre...