<i>sfi1-C4A</i> displays impaired growth and spindle and chromosome segregation defects.

<p><b>A</b>. Dilution series of strains with mutations in <i>SFI1</i> on YPD. <b>B–C</b>. Immunofluorescent staining (α-tubulin: green, DNA: blue) of fixed large-budded <i>sfi1-C4A</i> (JA249) or control (JA196) cells grown at 24°C or shifted to 37°C for 8.25 h in YPD. MT: microtubule, SS: short metaphase bipolar spindle, BPS/SP: bipolar spindle or separated poles. Bar: 5 µm. <b>C</b>. Quantification of B. Asterisks: statistically significant difference via Student's <i>t</i> test between <i>sfi1-C4A</i> and control for each phenotypic category at 24°C (red) and 37°C (black). **p<0.01. *: p<0.05. Significance only shown for comparisons between strains at each temperature. Error bars: SD. n≥192 cells per group from 2 experiments. <b>D–E</b>. <i>GFP-sfi1-C4A pLEU-HIS-SPC42-mCherry</i> (JA354) or <i>GFP-SFI1 pLEU-HIS-SPC42-mCherry</i> (JA311) grown in SC-Leu at 24°C or shifted to 37°C 9 h were fixed prior to localization (D) of Sfi1 (green) and Spc42 (red) and quantification of Sfi1 levels in arbitrary units (a.u.) (E). Error bars: SD. n≥62 cells per group from 2 experiments. Bar: 5 µm.