Multiple sirtuins permit growth of cells lacking Yku70/80.

<p>(A-B) <i>yku70Δ</i> causes synthetic growth defects when combined with <i>hst3Δ hst4Δ</i>. Five-fold serial dilution of cells were spotted on solid media and incubated at 25°C. (B) Doubling time for strains in A incubated in YPD at 30°C (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007356#sec009" target="_blank">material and methods</a>). Error bars: standard deviation, p-value = 7.42x10^-7 (two-sided student’s T-test). (C-D) Preventing H4K16 acetylation rescues the growth of <i>yku70Δ</i> mutants in NAM. Yeast cells were incubated in a 96-well plate containing increasing concentrations of NAM. OD₆₃₀ readings were acquired after 48 h at 30°C and results were normalized to untreated controls. Error bars: standard deviation. (E) Deletion of genes encoding subunits of the Hst1-Sum1-Rfm1 complex causes synthetic lethality when combined with <i>hst3Δ hst4Δ yku70Δ</i>. (F) Lack of Sum1 causes synthetic sensitivity to MMS-induced replicative stress when combined <i>yku70Δ</i>. (G-H) H4K16ac is deleterious to the growth of <i>yku70Δ</i> mutants in the presence of MMS-induced replicative stress or at elevated temperatures.