Effect of free chelatable zinc on hypobaric hypoxia induced alteration in Bax/Bcl-2 expression.

<p>Graph represents the relative mRNA expression of Bax/Bcl-2 in the hippocampus (A). Lane 1-Normoxia, Lane 2-Normoxia treated with Ca₂EDTA, Lane 3- Hypoxia and Lane 4- Hypoxia treated with Ca₂EDTA. Graph (B) represents the relative protein expression of Bax/Bcl-2 in the hippocampus. Lane 1-Normoxia, Lane 2-Normoxia treated with Ca₂EDTA, Lane 3- Hypoxia and Lane 4- Hypoxia treated with Ca₂EDTA. *, # indicate p<0.05. ***, ### indicate p<0.001. * compared hypoxia vs normoxia and normoxia treated Ca₂EDTA. # compared hypoxia treated Ca₂EDTA vs hypoxia. N- Normoxia, NED-Normoxia Treated with Ca₂EDTA, IH-Hypoxia and HED-Hypoxia Treated with Ca₂EDTA. Bcl-2: Forward -5′-CTGGCATCTTCTCCTTCCAG-3′; Reverse - 5′-GACGGTAGCGACGAGAGAAG-3′ (183 bp); Bax: Forward: 5′-TGAAGACAGGGGCCTTTTTG-3′; Reverse: 5′-AATTCGCCGGAGACACTCG-3′ (139 bp).