Dynamin inhibition affects secretion and degradation of pre-labeled [³⁵S]-apoE.

<p>HMDM were incubated in methionine/cysteine free DMEM with 250 µCi/mL [³⁵S]methionine/cysteine for 3 h and received a preincubation with or without 30 µM MiTMAB during the last 30 min. Cells were then washed and chased in medium containing unlabelled methionine/cysteine, without or with 30 µM MiTMAB. At the indicated times [³⁵S]-labelled apoE was immunoprecipitated from media and cell lysates, separated by SDS-PAGE and quantified by phosphorimaging. Secreted [³⁵S]-apoE (A); cell-associated [³⁵S]-apoE (B); net degradation of [³⁵S]-apoE (C), calculated by subtracting residual [³⁵S]-apoE in cell and media from [³⁵S]-apoE at T₀. Inserts show representative images. Fitted data according to Eq1 are shown (D–F). Symbols Ctrl (^○) and MiTMAB (•) represent experimental data. *<i>P</i><0.05 between treatments (Repeated Measures 2-way ANOVA).