Identification of the mAb 3D7 epitope by Western blot.

<p>Western blot analysis of GST-HuN4-F112/F5-N fusion proteins with the mAb 3D7 (A). Lane 1: ultracentrifugal HuN4-F112; lane 2: GST-HuN4-F112-N; lane 3: GST-HuN4-F5-N; lane 4: GST. Truncated fragments were detected with the mAb 3D7 (B). The mAb 3D7 specifically reacted with N protein fragment NF1-3-2 (amino acids 7–33) after three rounds of truncation. F1B is the fragment (amino acids 10–33) identified previously as not recognized by the mAb 3D7 (data not shown), used here as a negative control.