Identification of the epitopes recognised by the two mAbs by Western blot.

<p><b>A</b>: Several peptide epitopes from NogoA were produced. A library of six Nogo deletion constructs was made, and GST-tagged recombinant proteins were designed. <b>B</b>: Lysates of induced BL21 bacteria with recombinant plasmids were analysed using Coomassie brilliant blue staining (lanes from left to right: lysates of induced BL21 bacteria with recombinant plasmids GST-ΔNogoA-Na, GST-ΔNogoA-Nb, GST-ΔNogoA-Nc, GST-ΔNogoA-Nd, GST-ΔNogo66a, and GST-ΔNogo66b; lane 7: lysate of induced BL21 bacteria with empty pGEX-4T-1 vector; lane 8: lysates of non-induced BL21 bacteria alone). <b>C</b>: Recombinant proteins identified by WB. Anti-GST antibody was used to detect the fragments (lanes from left to right: the recombinant proteins GST-ΔNogoA-Na, GST-ΔNogoA-Nb, GST-ΔNogoA-Nc, GST-ΔNogoA-Nd, GST-ΔNogo66a, and GST-ΔNogo66b; lane 7: GST protein; lane 8: non-induced). <b>D</b>: The epitope recognised by the aNogoA-N mAb (lanes from left to right: the recombinant proteins GST-ΔNogoA-Na, GST-ΔNogoA-Nb, GST-ΔNogoA-Nc, GST-ΔNogoA-Nd and GST protein). <b>E</b>: The epitope recognised by the aNogo66 mAb (lanes from left to right: the recombinant proteins GST-ΔNogo66a and GST-ΔNogo66b and GST protein).