<p>Primers that have been used to generate GFP- miRNA target gene constructs. Restriction sites are highlighted in bold letters.</p
<p>These plasmids were verified by DNA sequencing. Underlined nucleotides indicate the designated si...
<p>Bold denotes added restriction sites and italics denote spacer nucleotides added to facilitate pr...
a<p>M = A or C; N = A, C, G or T/U; Y = C or T; D = not C.</p>b<p>Nucleotides in bold represent the ...
<p>Restriction sites are shown in bold face and genome complementary regions in italics.</p
<p>Restriction enzyme sites generated by site-directed mutagenesis or by PCR are boldfaced and under...
<p>F: forward; R: reverse</p><p>* Uderlines indicate the restriction sites designed in the indicated...
<p>a. Introduced restriction sites are underlined. Amino acid spacer (GGGS) at the N-terminus of GFP...
<p>The bold values in the primer sequences represent restriction sites (OMP-DH forward-primer: PvuII...
<p>Restriction enzyme sites are underlined.</p><p>Additional nucleotides added to facilitate restric...
<p>The underlined bases correspond to the restriction sites included to aid in the subsequent clonin...
<p>The PCR primers are listed in pairs, with the forward primer (F) listed first and the reverse pri...
<p>Restriction site used in cloning is underlined.</p><p>Primers used in this study.</p
1<p>Underlined letters highlight engineered restriction enzyme (RE) sites (names of the introduced R...
<p>All PCR primers shown here were designed for this study.</p>a<p>Restriction sites are underlined....
<p>(Restriction sites are underlined.)</p><p>Primers used for PCR amplification.</p
<p>These plasmids were verified by DNA sequencing. Underlined nucleotides indicate the designated si...
<p>Bold denotes added restriction sites and italics denote spacer nucleotides added to facilitate pr...
a<p>M = A or C; N = A, C, G or T/U; Y = C or T; D = not C.</p>b<p>Nucleotides in bold represent the ...
<p>Restriction sites are shown in bold face and genome complementary regions in italics.</p
<p>Restriction enzyme sites generated by site-directed mutagenesis or by PCR are boldfaced and under...
<p>F: forward; R: reverse</p><p>* Uderlines indicate the restriction sites designed in the indicated...
<p>a. Introduced restriction sites are underlined. Amino acid spacer (GGGS) at the N-terminus of GFP...
<p>The bold values in the primer sequences represent restriction sites (OMP-DH forward-primer: PvuII...
<p>Restriction enzyme sites are underlined.</p><p>Additional nucleotides added to facilitate restric...
<p>The underlined bases correspond to the restriction sites included to aid in the subsequent clonin...
<p>The PCR primers are listed in pairs, with the forward primer (F) listed first and the reverse pri...
<p>Restriction site used in cloning is underlined.</p><p>Primers used in this study.</p
1<p>Underlined letters highlight engineered restriction enzyme (RE) sites (names of the introduced R...
<p>All PCR primers shown here were designed for this study.</p>a<p>Restriction sites are underlined....
<p>(Restriction sites are underlined.)</p><p>Primers used for PCR amplification.</p
<p>These plasmids were verified by DNA sequencing. Underlined nucleotides indicate the designated si...
<p>Bold denotes added restriction sites and italics denote spacer nucleotides added to facilitate pr...
a<p>M = A or C; N = A, C, G or T/U; Y = C or T; D = not C.</p>b<p>Nucleotides in bold represent the ...