Phagocytosis of <i>E. coli</i> bioparticles by haemocytes from uninfected and <i>Trichobilharzia regenti</i> infected <i>Radix lagotis</i>.

<p>Phagocytic activities were assessed by incubating <i>E. coli</i> bioparticles with haemocyte monolayers and assessing the relative fluorescence of internalised particles after 2 h using a microplate reader. (A) The combined (phase-contrast and fluorescence) image of <i>E. coli</i> bioparticles (a) within a haemocyte (b); scale bar = 10 µm. (B) Data were evaluated per volume of haemolymph (200 µl) and per number of haemocytes (50,000) (shown as mean values ± SEM; n = 7) with uninfected snails considered as having 100% activity. *p<0.05 when compared to uninfected snails (50,000 haemocytes); Wilcoxon test.