Basal H₂O₂ production in haemocytes from uninfected and <i>Trichobilharzia regenti</i> infected <i>Radix lagotis</i>.

<p>H₂O₂ output by haemocyte monolayers was detected by Amplex red and the intensity of fluorescence measured by microplate reader over 60 min. The mean relative fluorescence values are shown (± SEM; n = 7) and represent the increase in H₂O₂ production over time. Data were evaluated per volume of haemolymph (50 µl) and per number of haemocytes (50,000). *p<0.05, **p<0.01, when compared to infected snails (50,000 haemocytes); two-sample t-test or Wilcoxon test combined with Fishers's combination test.