Diode array rapid scanning spectra for the intermediates and corrin ring destruction by reacting (CN)₂-Cbi with HOCl at three sequential time frames.

<p>Panel A, spectra traces collected at 0.0, 0.4, 0.8, 1.2, 1.6, and 2.4 s and was attributed to the replacement of the first molecule of CN^- with OCl in (CN)₂-Cbi. Panel B, spectra traces collected at 2.4, 5.0, 7.4, and 12.0 and were attributed to the replacement of the second molecule of CN^- with OCl in (CN)₂-Cbi. Panel C, spectra collected at 12.0, 22.0, 40.0, and 120.0 s and was attributed to corrin ring destruction. Experiments were carried out by rapid mixing a phosphate buffer solution (200 mM, pH 7.0), at 25°C, supplemented with 20 µM (CN)₂-Cbi with a same volume of a buffer solution supplemented with 80-fold excess of HOCl. Arrows indicate the direction of spectral change over time as each intermediate advanced to the next. These data are representative of three independent experiments.