The relative responsiveness of the wild type (WT) and selected rP2X4R mutants to P2XR agonist analogs.

<p>ATP and agonists were applied in 100 µM concentrations for 2 s with a washing interval of 60 s. The data are the mean ± SEM, relative to ATP efficacy (100%), from 26 to 37 measurements for the WT receptor and from 3 to 18 measurements per mutant. <i>Group I:</i> Mutants that exhibited changes in ATP potency/efficacy, deactivation kinetics, and changes in the relative responsiveness to orthosteric analog agonists. <i>Group II:</i> Mutants that exhibited changes in ATP potency/efficacy and deactivation, but no changes in the relative responsiveness to analog agonists. <i>Group III:</i> Mutants that showed no significant changes in ATP potency/efficacy. The statistical significance was determined by an ANOVA comparing the responsiveness to agonists between WT and mutant receptors: **, p<0.01, *, p<0.05. DF, Dorsal Fin; LF, Left Flipper.</p><p>The relative responsiveness of the wild type (WT) and selected rP2X4R mutants to P2XR agonist analogs.