Alanine- and cysteine-scanning mutagenesis of the V47–V61 rP2X4R segment.

<p>In this and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059411#pone-0059411-t002" target="_blank">Table 2</a>, the potency is expressed as the ATP concentration producing 50% of the maximal response (EC₅₀), and the efficacy as the maximum induced current (I_max) in response to 100 µM ATP. The I_max data are expressed as the mean ± SEM from 12–40 measurements per mutant and 96 measurements from the wild type (WT) receptor. n.d., not determined; n.f., nonfunctional;</p>**<p>p<0.01, between mutant and WT (used for I_max measurements);</p>*<p>p<0.05 between mutant and WT (used for EC₅₀ estimation).