RBD-specific antibody response in immunised mice.

<p>A) Total RBD-specific IgG titres were determined by ELISA 2 weeks after the second and third immunisations. B) NaSCN-displacement ELISA to measure the avidity of RBD-specific IgG 2 weeks after the third immunisation. Median displacement is shown for each group against an increasing concentration of NaSCN. The level of NaSCN required to displace 50% of the IgG is indicated. C, D, E and F) Total IgG1, IgG2a and IgG2b RBD-specific titres were determined by ELISA 2 weeks after the third immunisation. Each group contained 8 mice and all experiments repeated for 3 times.