Delocalisation of E-cadherin and beta-catenin in primordium cells in absence of PrP2.

<p><b>A</b>. Schematic representation of <i>claudinB-GFP</i> embryo at 30 hpf, primordium structure, and transversal view of a rosette showing the level of confocal focus plan. <b>B–B″</b>. In control embryos at 30 hpf, E-cadherin is expressed at the membrane level at the basal side of the primordium. <b>C–C″.</b> In morphant embryos, E-cadherin is observed in the cytoplasm and cellular membrane at basal and apical levels. The staining pattern is more punctuated in morphant compared to control. <b>D, E.</b> In control embryos, beta catenin expression is observed at the membrane level and at the center of rosette structures. <b>F, G</b>. In morphant, small rounded primordium shows a membrane homogeneous pattern with no rosette.