SF pretreatment inhibited I/R inducedretina cell apoptosis.

<p>The apoptotic cells in the retina were stained using a TUNEL -kit as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114186#s2" target="_blank">Methods</a> section. (A-C, D-F) Representative micrographs of retinal sections obtained 24 h (A-C) or 7 days (D-F) after ischemia and stained for TUNEL. (G) Quantitative analysis of the TUNEL-positive cells in the retina (mean ± SEM, n = 5). Control: sham-operated animal, I/R: vehicle-treated animal with 1 h of ischemia, and SF+I/R: SF-pretreated animal with 1 h of ischemia. ** p<0.01, *** p<0.001 compared with control, ### p<0.001 compared with I/R within the same time point, two-way ANOVA with Bonferroni post hoc test. The conventions are the same as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114186#pone-0114186-g001" target="_blank">Figure 1</a>.