SF pretreatment inhibited the I/R induced decrease in the IRL thickness.

<p>H&E staining was performed to measure the IRL thickness at 7 days after ischemia. (<b>A</b>) Typical images of retinal sections from the middle area (7 days after retinal ischemia). (<b>B</b>) Quantitative analysis of the IRL thickness. (mean ± SEM, n = 5). Control: the non-ischemic rats, I/R: vehicle-treated rats with 1 h of ischemia, and SF+I/R: SF-pretreated rats with 1 h of ischemia. ** <i>p</i><0.01, *** <i>p</i><0.001 compared to control, ## <i>p</i><0.01 compared to I/R, one-way ANOVA with Bonfferoni post hoc test. The conventions are the same as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114186#pone-0114186-g001" target="_blank">Figure 1</a>.