T_CM and CD44⁺CD62L⁻Ly6C⁺ cells generate CD44⁺CD62L⁻Ly6C⁺Tbet⁺ cells during chronic infection.

<p>(<b>A–D</b>) Equivalent numbers of FACS sorted cells as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004538#ppat-1004538-g007" target="_blank">Figure 7</a> were adoptively transferred into infection matched congenic recipients. Fourteen days post-transfer purified CD4+ T cells from the dLN and spleens of recipient mice were analyzed for the presence of transferred cells by flow cytometry. (<b>A</b>) Representative concatenated dot plots of 2 dLNs gated on transferred cells. Numbers in quadrants represent frequency. (<b>B</b>) Analysis of the frequency of CD44⁺CD62L⁻Ly6C⁺Tbet⁺ cells within the proliferated or non-proliferated portion of the indicated transferred population. (<b>C</b>) Analysis of the absolute number of total, proliferated, or non-proliferated CD44⁺CD62L⁻Ly6C⁺Tbet⁺ cells per organ or per mouse (dLN+SPL) derived from the indicated transferred population. Data in (B) is pooled from 3 independent experiments employing a total of 4–6 mice per group. Data in (C) is pooled from 2 independent experiments in which counting beads were employed to determine the absolute number of cells employing a total of 3–4 mice per group.