Adoptive transfer of T cell populations into MAP infected <i>Rag2</i>^−/− mice.

<p><b>A.</b> Sorting strategy and reanalysis of CD4⁺CD45RB^lo/int, CD4⁺CD45RB^hi and CD8⁺ T cells from spleen of naïve mice. Gates were set to sort pure population of CD3⁺CD19⁻CD4⁺CD45RB^lo/int, CD19⁻CD3⁺CD8⁻CD4⁺CD45RB^hi, CD3⁺CD19⁻CD4⁻CD8⁺ T cells. <b>B</b>. Analysis of reconstituted mice for CD4⁺ and CD8⁺ T cells four weeks after transfer i.e. day 56 of the experiment. Ctr – control; Inf – only infected with MAP at the beginning of the experiment, Rec – reconstituted with the T cell population indicated; Inf & Rec – infected at the beginning of the experiment and reconstituted after 4 weeks.