Immunohistochemistry (IHC) staining for localization of <i>Y. pestis</i> in the organs collected from immunized group animals on 3^rd and 20^th day post infection with <i>Y. pestis</i> and the naive control animals that were neither immunized nor challenged.

<p>The F1 antigen of <i>Y. pestis</i> was identified with anti-mouse FITC conjugated secondary antibody in the tissue sections collected from immunized animal groups on 3^rd day post infection including naive control <i>i.e.</i>, Naive control (A); PBS control (B); HSP70(II) (C); F1 (D); F1+HSP70(II) (E); LcrV (F); LcrV+HSP70(II) (G); F1+LcrV (H); F1+LcrV+HSP70(II) (I). Tissue sections were collected from the survived animal groups on 20^th day post infection <i>i.e.</i>, LcrV (J); LcrV+HSP70(II) (K); F1+LcrV (L); F1+LcrV+HSP70(II) (M). Fluorescent images representing the localization of <i>Y. pestis</i> in tissue sections of Lung [<b>a</b>]; Spleen [<b>b</b>]; Kidney [<b>c</b>]; and Liver [<b>d</b>].