<p>The mutations introduced into the binding sites are italicized and in lowercase.</p><p>Primers used for cloning of promoter-luciferase constructs, PCR-based site-directed mutagenesis, real-time PCR and ChIP.</p
<p>The PCR primers are listed in pairs, with the forward primer (F) listed first and the reverse pri...
<p>*Restriction sites are in bold; primer regions that anneal to 2A<sup>pro</sup> gene are underline...
<p>*The T7 promoter regions are underlined and the restriction enzyme sites are italicized.</p
<p>Primers used for cloning, site-directed mutagenesis, and sequence analysis.</p
<p>*The bold and underlined letters denote the mutated sequences.</p><p>Primers used for site-direct...
<p>Primers used for making CYP2E1 promoter luciferase reporter gene constructs.</p
<p>Primers used for mutant construction, complementation and RT-PCR analyses.</p
<p>These plasmids were verified by DNA sequencing. Underlined nucleotides indicate the designated si...
<p>Primers used for gene cloning, checking morpholino efficacy and site-directed mutagenesis.</p
<p>Positions corresponding to the predicted transcription factor binding sites are underlined. Mutat...
<p>Primers used for cloning of P domain and site-directed mutagenesis to generate single mutation in...
<p>Restriction site targets introduced to allow sequential cloning of the PCR-amplified fragments ar...
<p>Primer sequences used to obtain the transforming constructs and to identify mutants by PCR and So...
<p><i>Nde</i>I and <i>Xho</i>I restriction sites are underlined in forward and reverse cloning prime...
<p>Primers used for 5’ flanking sequence cloning, deletion mutant construction, EMSA, mRNA construct...
<p>The PCR primers are listed in pairs, with the forward primer (F) listed first and the reverse pri...
<p>*Restriction sites are in bold; primer regions that anneal to 2A<sup>pro</sup> gene are underline...
<p>*The T7 promoter regions are underlined and the restriction enzyme sites are italicized.</p
<p>Primers used for cloning, site-directed mutagenesis, and sequence analysis.</p
<p>*The bold and underlined letters denote the mutated sequences.</p><p>Primers used for site-direct...
<p>Primers used for making CYP2E1 promoter luciferase reporter gene constructs.</p
<p>Primers used for mutant construction, complementation and RT-PCR analyses.</p
<p>These plasmids were verified by DNA sequencing. Underlined nucleotides indicate the designated si...
<p>Primers used for gene cloning, checking morpholino efficacy and site-directed mutagenesis.</p
<p>Positions corresponding to the predicted transcription factor binding sites are underlined. Mutat...
<p>Primers used for cloning of P domain and site-directed mutagenesis to generate single mutation in...
<p>Restriction site targets introduced to allow sequential cloning of the PCR-amplified fragments ar...
<p>Primer sequences used to obtain the transforming constructs and to identify mutants by PCR and So...
<p><i>Nde</i>I and <i>Xho</i>I restriction sites are underlined in forward and reverse cloning prime...
<p>Primers used for 5’ flanking sequence cloning, deletion mutant construction, EMSA, mRNA construct...
<p>The PCR primers are listed in pairs, with the forward primer (F) listed first and the reverse pri...
<p>*Restriction sites are in bold; primer regions that anneal to 2A<sup>pro</sup> gene are underline...
<p>*The T7 promoter regions are underlined and the restriction enzyme sites are italicized.</p
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