<p>(a) Fluorescence image of hippocampal neuron expressing Sept7-GFP and CD4-mRFP for 2 weeks (DIV7+14), inset is showing spines that were selected for FRAP analysis. Scale bar is 5 µm. (b) Graph of CD4-mRFP, F-mRFP, GPI-mRFP and mRFP fluorescence-recovery halftimes in Sept7-GFP positive (red) and Sept7-GFP negative (green) spines. All experiments were done in ≥3 neurons in ≥10 spines. **p<0.01, Mann-Whitney test. (c, d) Maximum projection of movies of CD4-mRFP coupled QDs on neurons transfected with control RNAi (c) or Sept7 RNAi (d), illustrating the total area browsed by diffusing molecules (red). The CD4-mRFP staining of the cell is shown in green. Note that on Sept7 RNAi treated neurons QDs move great distances and browse many spines. ...
Excitatory glutamatergic synapses at dendritic spines exchange and modulate their receptor content v...
The purpose of this study was to examine whether variability in the shape of dendritic spines affect...
<p>(A) Schematic of the imaging of the cortex using the two-photon carrier. (B) Locating the observa...
<p>(a) Fluorescence images of fixed primary hippocampal neurons at various days in vitro (DIV) expre...
<p>(A) To investigate the dynamics of NESH in a single spine, the FRAP (fluorescence recovery after ...
<p>(a–c) Electronmicrographs showing the neuropil of CA1 stratum radiatum; spines (asterisks) and de...
<p>Brain slices from GIT1<sup>flox/flox</sup> mice transfected with GFP alone or GFP with tdTomato-C...
<p>(<b>A</b>) The WT and KO neurons were transfected with the GFP plasmid on DIV14 and fixed on DIV1...
<p><b>A</b>–<b>D</b>: Fluorescence photomicrographs demonstrating immunoreactivity against N protein...
<p>(A, B) Lentiviral vectors employed for co-expressing eGFP and miRNAs (A) and for expressing tdTom...
<p>FRAP analysis was performed to examine the effect of F-actin stabilization on NESH mobility. Hipp...
The purpose of this study was to examine whether variability in the shape of dendritic spines affect...
<p>Comparison of the recovery-halftimes in FRAP of transmembrane (Cd4-mRFP), inner membrane leaflet ...
<p>(A) Confocal image showing an example of a 17 DIV cultured hippocampal neuron transfected with mo...
<p>(a) Cultured hippocampal neurons were infected with lenti-virus encoding green fluorescent protei...
Excitatory glutamatergic synapses at dendritic spines exchange and modulate their receptor content v...
The purpose of this study was to examine whether variability in the shape of dendritic spines affect...
<p>(A) Schematic of the imaging of the cortex using the two-photon carrier. (B) Locating the observa...
<p>(a) Fluorescence images of fixed primary hippocampal neurons at various days in vitro (DIV) expre...
<p>(A) To investigate the dynamics of NESH in a single spine, the FRAP (fluorescence recovery after ...
<p>(a–c) Electronmicrographs showing the neuropil of CA1 stratum radiatum; spines (asterisks) and de...
<p>Brain slices from GIT1<sup>flox/flox</sup> mice transfected with GFP alone or GFP with tdTomato-C...
<p>(<b>A</b>) The WT and KO neurons were transfected with the GFP plasmid on DIV14 and fixed on DIV1...
<p><b>A</b>–<b>D</b>: Fluorescence photomicrographs demonstrating immunoreactivity against N protein...
<p>(A, B) Lentiviral vectors employed for co-expressing eGFP and miRNAs (A) and for expressing tdTom...
<p>FRAP analysis was performed to examine the effect of F-actin stabilization on NESH mobility. Hipp...
The purpose of this study was to examine whether variability in the shape of dendritic spines affect...
<p>Comparison of the recovery-halftimes in FRAP of transmembrane (Cd4-mRFP), inner membrane leaflet ...
<p>(A) Confocal image showing an example of a 17 DIV cultured hippocampal neuron transfected with mo...
<p>(a) Cultured hippocampal neurons were infected with lenti-virus encoding green fluorescent protei...
Excitatory glutamatergic synapses at dendritic spines exchange and modulate their receptor content v...
The purpose of this study was to examine whether variability in the shape of dendritic spines affect...
<p>(A) Schematic of the imaging of the cortex using the two-photon carrier. (B) Locating the observa...