Transient aneuploid and persistent diploid progeny are generated from α-α unisexual reproduction.

<p>A) The three panels show the read depths of all chromosomes in the three parental strains: 431α, XL280αSS, and XL280<b>a</b>. For each panel, the x-axis indicates the 14 chromosomes and the y-axis indicates the read depth from the Illumina sequencing. These strains had only one copy of chromosome 4, as no heterozygosity was detected for any marker from chromosome 4 that was analyzed, and thus, all are haploid. This was also consistent with results from the FACS analyses (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004849#pgen.1004849.s001" target="_blank">S1 Figure</a>). B) The six panels show the read depths of all chromosomes in strains derived from three meiotic progeny from α-α unisexual reproduction between strains 431α and XL280αSS. For each panel, the x-axis indicates the 14 chromosomes and the y-axis indicates the read depth from the Illumina sequencing. All of these strains showed heterozygosity at multiple markers along chromosome 4 (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004849#pgen-1004849-g006" target="_blank">Fig. 6</a>), indicating they had at least two copies of chromosome 4. FACS analyses indicated that these strains were likely diploid (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004849#pgen.1004849.s001" target="_blank">S1 Figure</a>), which was consistent with the sequencing results shown here suggesting all of the meiotic progeny had two copies for the majority of the chromosomes (including chromosome 4). The two panels within each of the three dashed-lined rectangles are sequencing results of small and large colonies, respectively, that were derived from the same meiotic progeny. C) The distribution of SNPs along chromosome 14 in the small and large colonies of the progeny SSB309, as well as the two parental strains 431α and XL280αSS. The genome sequence of each isolate was mapped against the published genome sequence of strain JEC21. The light blue bars indicate SNPs compared to JEC21, dark blue bars indicate heterozygous sites, while grey areas indicate regions that are identical to JEC21. The centromeric region of chromosome 14 is highlighted by the green rectangle.