Binding between apoB and apoD is disturbed by detergents and is dependent on the immobilized antigen.

<p>Finger blood plasma was treated with or without detergent before being added to ELISA plates. A) anti-apoD (D544) was used as the coating antibody, allowing for multiple apoD bonds to each LDL particle, as detected using anti-apoB (LDL20-biotin). B) LDL20 was used as the coating antibody, and biotinylated D544 was used as the detection antibody. The means ± SD of four replicates are shown. Experiments were repeated three times with same donor.