ELISA analysis of PAT-SM6 interactions.

<p>Assays were performed using (A) LDL or (B) oxidized LDL at coating concentrations of 0 µg/mL (open circles), 1 µg/mL (closed triangles), 2 µg/mL (open diamonds), 3 µg/mL (closed squares), 4 µg/mL (open inverted triangles), and 5 µg/mL (closed circles). (C) The data were fitted to a simple binding isotherm to obtain apparent binding constants (K_a) for PAT-SM6 binding as a function of the coating concentration of LDL (open circles) or oxidized LDL (closed circles). Error bars represent the 95% confidence intervals from the fitting of the ELISA data to a Langmuir binding curve.