Subcutaneous infection of BALB/c mice with either amastigote (A, D) or promastigote (B, C, E) <i>L. major</i>.

<p>A. Mice were infected with either 10⁵ wild type <i>L. major</i> (squares) or 10⁵<i>L. major</i> NTPD1 null mutant (triangles) amastigotes and lesion scores monitored weekly. Error bars represent S.E.M. (<i>n</i> = 5). No significant difference in lesion size was observed at any time point (<i>P</i>>0.05, unpaired t-test). B. Mice were infected with either 10⁶ wild type <i>L. major</i> (squares) or 10⁶<i>L. major</i> NTPD1 null mutant (triangles) parasites and lesion scores monitored weekly. Error bars represent S.E.M. (<i>n</i> = 10). Significant differences in lesion size were observed at all time points from week 6 inclusive (<i>P</i><0.05, unpaired t-test). C. Mice were infected with either 10⁶ wild type <i>L. major</i> + pIR1SAT (squares), 10⁶<i>L. major</i> NTPD1 null mutant + pIR1SAT (closed triangles) or 10⁶<i>L. major</i> NTPD1 null mutant + pIR1SAT-<i>ntpd1</i> (open triangles). Error bars represent S.E.M. (<i>n</i> = 5). D and E. Mice were infected with either 10⁵ wild type <i>L. major</i> (squares) or 10⁵<i>L. major</i> NTPD2 null mutant (circles) parasites and lesion scores monitored weekly. Error bars represent S.E.M. (<i>n</i> = 5). No significant difference in lesion size was observed between strains at any individual time point (<i>P</i>>0.05, two-way ANOVA).