Recruitment of T_reg cells into spleen, lung draining lymph node, and lung with CD4⁺Foxp3⁺T cell adoptive transfer during asthma induction in mice.

<p>The lymphocytes were isolated from spleen and lung draining lymph node (A). After staining, the lymphocytes were firstly gated with CD4 and the percentage of CD25+Foxp3+ cells calculated using FACS analysis. Paraffin sections of the lung from all of the mice in each group receiving CD4⁺Foxp3⁺T cells (green) were immunofluorescently stained for CTLA-4 (red) and nuclei (DAPI, blue), representative pictures are shown, (white bar = 100 µm) (B). Population of CTLA4⁺Foxp3⁺ cells of each group were analyzed in the total lung cells based on analysis the <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0003410#pntd.0003410.s004" target="_blank">S4 Fig</a>. using Image J program(C). (The value in grape represented the number of the CTLA4⁺ Foxp3⁺ cells per 1×10⁵ DAPI positive cells) [a; OVA-IV(inf):+(-) group, b; OVA-IV(inf):+(+) group, c; OVA+IV(inf):+(-) group, d; OVA+IV(inf):+(+) group. Merge; merge of CTLA-4, GFP, and DAPI filed screens, Mag-Merge; magnification of Merge, Sp; spleen, LLN, lung draining lymph node, OVA-; PBS treated mice, OVA+; allergic airway inflammation-induced mice, IV(inf)+(-); CD4⁺Foxp3⁺T cell of normal mice adoptive transferred mice, IV(inf)+(+); CD4⁺Foxp3⁺T cell of <i>T. spiralis-infected</i> mice adoptive transferred mice, *<i>p</i><0.05, **<i>p</i><0.01, <i>n</i> = 6 mice/group, 3 independent experiments].