Selective inhibition of TCA FIV infectivity by heparin.

<p>(A) Productive infection assay of FIV-PPRcr inhibited by heparin in G355-5 and Gfox cells. (B) Productive infection assay of FIV-34TF10 in G355-5 and FIV-PPR in Gfox cells inhibited by heparin. Virus growth in cells was evaluated as CPM by a reverse transcriptase activity assay over time. Heparin was used at 20 µg/ml. Results are means and standard deviations (SD) for three independent determinations. ***p<0.001; **p<0.01; *p<0.05; as compared to the untreated control group, (C). Effect of heparin on FIV TCA entering G355-5 cells and FS entering Gfox cells. Entry assay were performed in the presence or absence of heparin at indicated concentrations. Values are inhibition percentage calculated as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115252#s4" target="_blank">Materials and Methods</a>”. Results are means and standard deviations (SD) for three independent determinations. ***p<0.001; **p<0.01; *p<0.05; as compared to the FIV TCA groups.