Inhibitory effect of heparin on attachment of MAV-1 and Ad5 to A549 cells.

<p>Various concentrations of heparin were pre-incubated either with cells or with virus during 1 hour at 37°C. Pretreated cells were infected with virus (3×10³ particles per cell) during 1 hour at 4°C. In parallel, untreated cells were chilled on ice and infected with virus (final multiplicity of infection: 3×10³ particles per cell) that had been pre-incubated with heparin. Subsequently, non-bound virus particles were washed away and cells were subjected to DNA-extraction for analysis by real-time PCR. Binding capacities are presented as the percentage attached virus particles relative to the total amount of added virus particles. Means (±SEM) of three independent experiments are shown (each condition performed in duplicate). *, <i>P</i><0.05 <i>versus</i> 0 mg/ml heparin.