PP2A activity is increased in the absence of NOSIP.

<p>(A) Protein levels in MEFs from WT and NOSIP KO mice were analysed by IB with antibodies against PP2Ac, the methylated form of PP2Ac (α-PP2Ac-m), LCMT, PME, PTPA, alpha4 and NOSIP. IB using α-vinculin is shown as loading control. (B) PP2A activity was measured in MEFs isolated from WT, HET and NOSIP KO mice. Values are means ± SEM, n = 6; statistical significance was analysed by One-way ANOVA with Bonferroni post-test, 95% confidence interval (top). Following the activity measurement all samples were subjected to IB with α-PP2Ac to determine the amount of immunoprecipitated PP2A and the activity was normalised to relative intensity of α-PP2Ac IB. One representative experiment is shown (bottom). (C) PP2A activity was measured in lung, palate and midfacial tissue (excluding palate, maxilla and mandible) isolated from WT and NOSIP KO embryos at E18.5 backcrossed to C57Bl/6J for ten generations. Values are means ± SEM, n = 7; statistical significance was analysed by Wilcoxon matched pairs test, two tailed, 95% confidence interval (top). Following the activity measurement all samples were subjected to IB with α-PP2Ac to determine the amount of immunoprecipitated PP2A in the activity assay and one representative experiment is shown (bottom). Activity data are normalised to relative intensity of α-PP2Ac IB.