PP2A is a novel interacting protein of NOSIP.

<p>(A) PP2A subunits found to interact with NOSIP in an affinity chromatography approach followed by mass spectrometry analysis. (B) GST-NOSIP was used for GST-pulldown experiments using HeLa cell lysates endogenously expressing PP2A. 0.5% of the volume of the cell lysate used for pulldown is shown for comparison of protein levels (0.5% input). Proteins are detected by immunoblotting with PP2A subunit-specific antibodies. (C) Co-immunoprecipitation of endogenous PP2A subunits with endogenous NOSIP from HeLa cell lysates using a polyclonal NOSIP-specific antiserum for immunoprecipitation (IP). Precipitation with sepharose beads only served as specificity control. 0.2–0.8% of the volume of the cell lysate used for IP is shown for comparison of protein levels (% input). Proteins are detected by immunoblotting with PP2A subunit-specific antibodies and a NOSIP-specific antiserum.