miR-33b negatively regulates c-Myc gene expression.

<p>(A) The relative mRNA of c-Myc expression levels were determined by qRT-PCR in human osteoblast cell line (hFOB) and osteosarcoma cells (SOSP-9607, U2OS, MG-63, SAOS-2). (B) Western blot analysis of c-Myc expression in human osteoblast cell line (hFOB) and osteosarcoma cells (SOSP-9607, U2OS, MG-63, SAOS-2). (C) qRT-PCR analysis of c-Myc expression in 40 pairs osteosarcoma tissues and their corresponding adjacent normal bone tissues. The expression of c-Myc was normalized to GAPDH. The expression of c-Myc in osteosarcoma tissues was significant higher than in the corresponding adjacent normal bone tissues. (D) Analysis of correlation of miR-33b and c-Myc expression in osteosarcoma tissues. Student’s <i>t</i> test was used to analyze the significant differences between the osteosarcoma cells and osteoblast cell line. ***p<0.001.