c-Myc is required for miR-135b-directed osteosarcoma cell proliferation and invasion.

<p>(A) Western blot analysis of c-Myc in MG-63 cells that were co-transfected with either a miR-135b mimic (20 nM) or scrambled miRNA (20 nM) and either a pcDNA-c-Myc (2 µg) or pcDNA-empty vector (2 µg). (B) Cell proliferation assays with MG-63 cells that were treated as described in (A). (G) Trans-well analyses of MG-63 cells treated as described in (B). The relative ratio of the invasive cells per field is shown. (D) Western blot analyses of c-Myc protein expression in eight patients with miR-135b expression that was down-regulated in the osteosarcoma tissues (C) compared to the corresponding adjacent non-neoplastic tissues (N). The bars represent the mean±SD (*p<0.05, **p<0.01 and ***p<0.001).