Virus entry kinetics correlate with the stoichiometry of entry.

<p>(A) Entry kinetic curves for JR-FL wt and JR-FL ΔV1V2. Synchronized pseudovirus infection of TZM-bl cells following spinoculation was terminated by addition of T-20 at the indicated timepoints. Infectivity reached after 120 minutes was set as 100% and all data were normalized relative to this value. Data are mean and SD from 3 independent experiments. (B) Half maximal entry time for wt and V1V2-deleted envs was calculated from kinetic profiles shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004595#ppat-1004595-g004" target="_blank">Fig. 4A</a> and <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004595#ppat.1004595.s007" target="_blank">S7B Fig.</a> Time (in minutes) required to reach 50% entry into target cells is depicted. Data shown are means derived from 2 to 4 independent experiments. The p-value was calculated by a paired t-test. (C) Correlation analysis (Pearson) of wt (black symbols) and V1V2-deleted env (red symbols) half-maximal entry time and estimated T.