Sup35 aggregates initially appear near the vacuole, from which short lines extend to the periphery to form rings.

<p>Sup35NM-RFP was overexpressed from p2017 by growing [<i>PIN⁺</i>] BY4741 cells with endogenous <i>HSP42-GFP</i> in 2% Gal. Time shown is after the addition of 2% Gal (see details in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004814#pgen-1004814-t002" target="_blank">Table 2</a>).