<p>A ten-fold serial dilution of <i>rbp2</i> plasmid (1 to 100,000 copies/μl) is shown in the amplification plot. The cycle threshold was determined automatically by the ABI 7500 fast system software program. The negative control sample (red line) shows no amplification over the cycle threshold for 60 cycles.</p
<p>Serial dilutions of stock BCG (A) and H37RV (B) are represented on the X-axis with the C<sub>T</s...
<p><sup>1</sup> NAE, flat-glass Nucleic Acid Extraction cards (BCSI); Qiagen, Qiagen AllPrep Total N...
<p>a) The 430 bp cytb-qPCR products for each species. b-e) Four RFLP assays using FspBI, AluI, HpyCH...
A representative 1D amplitude plot of ddPCR reactions (A) and amplification curve of qPCR (B). ddPCR...
<p>A representative standard curve demonstrates linearity based on 10-fold serial dilutions (1 to 10...
A representative LOD and LOQ amplification curve of qPCR (A) and 1D amplitude plot of ddPCR reaction...
Quantification using ddPCR was shown in copy number due to its capability to perform absolute quanti...
<p>A typical amplification plot (a, d) and the corresponding standard curve (b, e) for the pLSUO and...
<p>Cycle threshold (Ct)-values generated by a specific qPCR assay for a ten-fold dilution series of ...
The tRNApyl and pylRSwt qPCR assays were tested with a tenfold serial dilution of their respective p...
<p>Plasmid DNA was tenfold diluted serially from 5.88E+6–5.88E+0 copies/μL. The slope of the plasmid...
<p>Repeatability and reproducibility of the <i>rbp2</i> plasmid standard curves determined via Cq va...
<p><b>(A) Amplification curve of qPCR</b> showing 10-fold serial dilution of the WSSV DNA standard p...
<p>A: Representative amplification plots of plasmid containing <i>JAK2</i> exon 12 mutation diluted ...
<p>Plasmid dilution series were used to create calibration curves for efficiency calculations. Top p...
<p>Serial dilutions of stock BCG (A) and H37RV (B) are represented on the X-axis with the C<sub>T</s...
<p><sup>1</sup> NAE, flat-glass Nucleic Acid Extraction cards (BCSI); Qiagen, Qiagen AllPrep Total N...
<p>a) The 430 bp cytb-qPCR products for each species. b-e) Four RFLP assays using FspBI, AluI, HpyCH...
A representative 1D amplitude plot of ddPCR reactions (A) and amplification curve of qPCR (B). ddPCR...
<p>A representative standard curve demonstrates linearity based on 10-fold serial dilutions (1 to 10...
A representative LOD and LOQ amplification curve of qPCR (A) and 1D amplitude plot of ddPCR reaction...
Quantification using ddPCR was shown in copy number due to its capability to perform absolute quanti...
<p>A typical amplification plot (a, d) and the corresponding standard curve (b, e) for the pLSUO and...
<p>Cycle threshold (Ct)-values generated by a specific qPCR assay for a ten-fold dilution series of ...
The tRNApyl and pylRSwt qPCR assays were tested with a tenfold serial dilution of their respective p...
<p>Plasmid DNA was tenfold diluted serially from 5.88E+6–5.88E+0 copies/μL. The slope of the plasmid...
<p>Repeatability and reproducibility of the <i>rbp2</i> plasmid standard curves determined via Cq va...
<p><b>(A) Amplification curve of qPCR</b> showing 10-fold serial dilution of the WSSV DNA standard p...
<p>A: Representative amplification plots of plasmid containing <i>JAK2</i> exon 12 mutation diluted ...
<p>Plasmid dilution series were used to create calibration curves for efficiency calculations. Top p...
<p>Serial dilutions of stock BCG (A) and H37RV (B) are represented on the X-axis with the C<sub>T</s...
<p><sup>1</sup> NAE, flat-glass Nucleic Acid Extraction cards (BCSI); Qiagen, Qiagen AllPrep Total N...
<p>a) The 430 bp cytb-qPCR products for each species. b-e) Four RFLP assays using FspBI, AluI, HpyCH...