Lower limits of detection of MVT and static qPCR.

<p>¹ NAE, flat-glass Nucleic Acid Extraction cards (BCSI); Qiagen, Qiagen AllPrep Total Nucleic Acid kit.</p><p>² TSB, trypticase soy broth; 7H9, Middlebrook 7H9 broth with ADC enrichment. All stimulations at 37°C except <i>A. hydrophila,</i> which was stimulated at 30°C.</p><p>³ LOD, limit of detection. The LOD of MVT was the lowest density of spiked bacteria to exhibit a positive result, defined as ΔCt <u>></u> 1 in three out of three replicates. MVT was always negative in the absence of spiked bacteria.</p><p>⁴ LOD, limit of detection. The LOD of qPCR was the lowest density of spiked bacteria to exhibit a positive result, defined as a stronger signal (lower Ct value) than all three no-bacteria controls, in three out of three replicates.</p><p>⁵ Experiment depicted graphically in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086433#pone-0086433-g001" target="_blank">Figure 1</a>.