GO analysis of <i>fkh2(6A)</i> and <i>fkh2ΔΔ</i> mutants.

<p>A) Venn diagram showing the number of genes down regulated in the <i>fkh2(6A)</i> and <i>fkh2ΔΔ</i> mutants and the overlap in the two data sets. B) Genes significantly more down regulated in <i>fkh2ΔΔ</i> mutant compared to the <i>fkh2(6A)</i> mutant and genes more down regulated in the <i>fkh2(6A)</i> mutant compared to <i>fkh2ΔΔ</i>. Each panel shows the genes down regulated at least two fold at the 5% FDR threshold in <i>fkh2(6A)</i> or <i>fkh2ΔΔ</i> (empirical Bayes moderated t-test). Genes significantly down regulated in <i>fkh2ΔΔ</i> (above) or <i>fkh2(6A)</i> (below) are highlighted in the solid points. Those genes significantly (5% FDR, empirical Bayes moderated t-tests) more down regulated in <i>fkh2ΔΔ</i> than <i>fkh2(6A)</i> are shown in blue (top panel), or more down regulated in <i>fkh2(6A)</i> than <i>fkh2ΔΔ</i> are shown in red (lower panel). Right: GO pathways enriched in genes in each category highlighted on the left. Note for reasons explained in the text the figures in this panel are not consistent with the Venn diagram shown in panel A. C) qPCR comparing the expression of hyphal associated transcripts in the Fkh2 phosphorylation mutants. Expression levels are normalised against <i>ADE2</i> and shown relative to the expression in the <i>fkh2/FKH2</i> strain. Means are from two independent biological repeats, each with three technical replicates. Vertical bars are equal to one standard error.