Overview of transcription data.

<p><b>A</b>) Summary of the number of significantly up and down regulated genes (FDR<0.01) observed across all six possible comparisons of the four sample types e.g. 463 genes are up regulated in KC/K whilst 53 genes are down regulated in KV/K. <b>B</b>) Venn diagram indicating the number of significant (FDR<0.01) differentially expressed genes across four key comparisons (KCV/KC, KC/K, KV/K and KCV/KC) and the overlap between each set of genes. <b>C–F</b>) Detailed analysis between the four comparisons illustrating the overlap between up and down regulated gene lists. Pairs of arrows in the intersection refer to the direction of fold change in the comparisons on the left and right hand sides respectively. Comparisons are shown for: <b>C</b>) KCV/KV and KCV/KV, <b>D</b>) KC/K and KCV/KC, <b>E</b>) KV/K and KCV/KC and F) KC/K and KCV/KV genes. For these four Venn diagrams (e.g comparison X vs. comparison Y), those genes that are up or down-regulated in X but not significantly altered in Y are shown on the left-hand side with single arrows denoting the direction of fold change. The converse is shown on the right-hand side (i.e. genes that are differentially expressed in Y but not in X), again with up or down arrows denoting the direction of fold change. The overlaps themselves show the 4 possible options: up in both X and Y, down in both X and Y, up in X and down in Y, or down in X and up in Y. These are denoted by pairs of arrows, with the left-hand arrow referring to the direction of fold change in X and the right-hand arrow denoting the direction of fold change in Y e.g. in KC/K vs. KCV/KC (<b>D</b>), 4 genes are up regulated in both comparisons, 2 are down regulated in both comparisons, 73 are up regulated in KC/K but down regulated in KCV/KC whilst 159 are down regulated in KC/K but up regulated in KCV/KC.